▣ Title : Plasma Physics and Chemistry for
Biological Cell Interactions
by Nonthermal Atmospheric Pressure Plasma
▣ Speaker
: Eun Ha Choi (Kwangwoon University)
▣ Date
& Time : Friday, June 13(2:00 ~ 3:30pm)
▣ Place
: LG Research Building, Room #101
▣ Host
: Prof. Jae Koo Lee (T.2083)
▣ Abstract :
Current
research activities of plasma bioscience and medicines in Plasma Bioscience
Research Center (PBRC), Korea, will be introduced along with the plasma physics
and chemistry for biological cell interactions. Basic diagnostics for electron
temperature and ion density have been introduced and measured to be ~ 1 eV and
~ 1×1013 cm-3, respectively, for the nonthermal atmospheric pressure dielectric barrier
discharge (DBD) plasma [1] and soft plasma jet [2] by atmospheric collisional
radiative model and wave-packet model[3]. Herein, we have also investigated the
basic generation mechanism of reactive oxygen species (ROS), especially for
hydroxyl radical OH species by ultraviolet absorption spectroscopy [4], and
their interactions with microbial [5] and mammalian cells resulting in
apoptotic cell death in accordance with the absolute densities of OH radical
species in biological solutions. Especially, differential selectivity of cell
death for epithelial human lung cancer cell H460 has been observed to be higher
than normal lung cell L132 in higher density of radical species, which is
caused by mitochondrial membrane potential decrease and enzymatic dysfunction
[1]. Similar effects of selective apoptosis were also observed with oral
squamous cell carcinoma, which the mechanism is suspected to be linked with
epidermal growth factor receptor (EGFR). Additionally, applications of
non-thermal plasma treatment on hard tissue regeneration have been considered
with regard to osteoblasts and mesenchymal stem cells. Recently, we also apply
DBD plasma and nitric oxide (NO) producing microwave plasma to enhance
differentiation and activation of microbial and mammalian cells. Furthermore,
we provide the possibility that nonthermal plasma jet could be developed as a
potential anti-diabetic therapy via ROS and NO dependent signaling pathway for
glucose uptake and insulin secretion [6].
References
[1]K. Pangomm, K. Y. Baik, M.K. Nam, J. H. Han, H. Rhim,
and E. H. Choi, Cell Death & Desease 4, e642 (2013).
[2]
P. Attri,
B. Arora, and E. H. Choi, RSC Advances 3, 12540 (2013).
[3]
G. Cho, E. H. Choi, and H. S. Uhm, Phys. Plasmas 18,
034504 (2011).
[4]
Y.J. Hong, C.J. Nam, K.B. Song, G.S. Cho, H.S. Uhm, D.I. Choi and E.H. Choi, JINST 7,C03046 (2012).
[5]
Y.H. Ryu,
Y.H. Kim, J.Y. Lee, G.B. Shim, H.S. Uhm, G. Park, and E.H. Choi, PLOS One 8,
e66231 (2013).
[6]
N. Kumar, N. K. Kaushik, G. Park, E. H. Choi, and H. S. Uhm,
Appl. Phys. Lett.
103, 203701 (2013).